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polyclonal rabbit anti mouse ifn α igg  (R&D Systems)


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    Structured Review

    R&D Systems polyclonal rabbit anti mouse ifn α igg
    Polyclonal Rabbit Anti Mouse Ifn α Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+mouse+ifn+%CE%B1+igg/Mouse+IFN-alpha+Antibody/pmc02738253-165-32-36
    Average 88 stars, based on 26 article reviews
    polyclonal rabbit anti mouse ifn α igg - by Bioz Stars, 2026-09
    88/100 stars

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    Related Articles

    Incubation:

    Article Title: Conventional Bone Marrow-Derived Dendritic Cells Contribute to Toll-Like Receptor-Independent Production of Alpha/Beta Interferon in Response to Inactivated Parapoxvirus Ovis
    Article Snippet: For intracellular IFN-α and IL-12/23p40 staining, BMDC were stimulated with iPPVO at an MOI of 5 as described above in the presence of GolgiPlug (BD) for the final 2 to 3 h. At least 10 6 cells were incubated in the presence of 25 μg of anti-CD16/CD32/ml for 10 min at 4°C, washed once in FACS buffer (3% FCS and 0.1% NaN 3 in PBS), and fixed in 4% formaldehyde in PBS. .. Subsequently, BMDC were incubated in permeabilization buffer (0.55% saponin, 5% FCS, and 2 mM HEPES in PBS) for 20 min and stained for intracellular IFN-α with the unlabeled monoclonal antibody RMMA-1 and polyclonal rabbit anti-mouse-IFN-α IgG (R&D Systems GmbH) or PE-labeled IL-12/23p40 (clone C15.6) as primary antibodies. .. The unlabeled anti-IFN-α antibodies were detected by using PE-labeled donkey anti-rat IgG F(ab′) 2 donkey anti-rabbit IgG F(ab′) 2 (2.5 μg/ml; Dianova).

    Staining:

    Article Title: Conventional Bone Marrow-Derived Dendritic Cells Contribute to Toll-Like Receptor-Independent Production of Alpha/Beta Interferon in Response to Inactivated Parapoxvirus Ovis
    Article Snippet: For intracellular IFN-α and IL-12/23p40 staining, BMDC were stimulated with iPPVO at an MOI of 5 as described above in the presence of GolgiPlug (BD) for the final 2 to 3 h. At least 10 6 cells were incubated in the presence of 25 μg of anti-CD16/CD32/ml for 10 min at 4°C, washed once in FACS buffer (3% FCS and 0.1% NaN 3 in PBS), and fixed in 4% formaldehyde in PBS. .. Subsequently, BMDC were incubated in permeabilization buffer (0.55% saponin, 5% FCS, and 2 mM HEPES in PBS) for 20 min and stained for intracellular IFN-α with the unlabeled monoclonal antibody RMMA-1 and polyclonal rabbit anti-mouse-IFN-α IgG (R&D Systems GmbH) or PE-labeled IL-12/23p40 (clone C15.6) as primary antibodies. .. The unlabeled anti-IFN-α antibodies were detected by using PE-labeled donkey anti-rat IgG F(ab′) 2 donkey anti-rabbit IgG F(ab′) 2 (2.5 μg/ml; Dianova).



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